Guía para el diseño y puesta en marcha de un plan de seguimiento de microalgas productoras de toxinas. Corporate author: IOC , IAEA . Person as. Palabras clave: alginato de calcio, Chlorella vulgaris, microalgas . La cinética de crecimiento se basó en el conteo de algas de cada cultivo, cada 24 horas.
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Effective harvesting of the microalgae Chlorella protothecoides via bioflocculation with cationic starch. In the present work, the flocculation efficiency of cationic starch Greenfloc was tested on the fresh water microalga Chlorella protothecoides under different conditions pH and flocculant concentrations. Different concentrations of Greenfloc 0, 2.
Dual-mode cultivation of Chlorella protothecoides applying inter-reactors gas transfer improves microalgae biodiesel production. Chlorella protothecoidesa lipid-producing microalgawas grown heterotrophically and autotrophically in separate reactors, the off-gases exiting the former being used to aerate the latter.
Autotrophic biomass productivity with the two-reactor association, 0. Fatty acid productivity was 1. This biomass’ fatty acid content was The carbon dioxide fixed by the autotrophic biomass was 45mgCO2L -1 h -1 in the symbiotic arrangement, 2. Thus, an increased efficiency in the glucose carbon source use and a higher environmental sustainability were achieved in microalgal biodiesel production using the proposed assembly.
Kinetics of Chlorella protothecoides microalgal oil using base catalyst. Directory of Open Access Journals Sweden. Full Text Available Due to continuous diminishing of fossil fuel resources and emission of greenhouse gases, the search for alternative fuels such as biodiesel and bioethanol has become inevitable. Biodiesel, also known as fatty acid methyl or ethyl ester, has emerged as a substitute for diesel because of similar fuel properties. Presently, biodiesel is produced from edible, non-edible and microalgal oil.
Chlorella protothecoides lipid content The present investigation shows the results of the kinetics of transesterification of C. The percentage of methyl ester yield is the only parameter chosen to carry out the optimum parameter and the kinetics of transesterification. The reaction rate constant was to be 0.
Furthermore, microalgal biodiesel is characterized for physico-chemical properties that are found to meet American ASTM D and Indian IS standards, especially in cold flow properties and stability of conventional biodiesel.
Microalgae such as Chlorella spp. Whole Algalin Protein WAP from dried milled Chlorella protothecoides was evaluated for dietary safety in a week feeding trial in rodents with genotoxic potential evaluated using in vitro and in vivo assays and the likelihood of food allergy potential evaluated via human repeat-insult patch test HRIPT. In the subchronic study, rats consumed feed containing 0, 25, 50, orppm WAP for days. No treatment-related mortalities or effects in general condition, body weight, food consumption, ophthalmology, urinalysis, hematology, clinical chemistry, gross pathology, organ weights, and histopathology occurred.
Several endpoints exhibited statistically significant effects, but none was dose-related. Cultivation of Chlorella protothecoides with urban wastewater in continuous photobioreactor: The capability to grow microalgae in nonsterilized wastewater is essential for an application of this technology in an actual industrial process. Batch experiments were carried out with the species in nonsterilized urban wastewater from local treatment plants to measure both the algal growth and the nutrient consumption.
Chlorella protothecoides showed a high specific growth rate about 1 day -1and no effects of bacterial contamination were observed. Different residence times were tested, and biomass productivity and nutrients removal were measured.
A maximum of microalgae productivity was found at around 0. Results from batch and continuous experiments were used to propose an integrated process scheme of wastewater treatment at industrial scale including a section with C. Sonication-based isolation and enrichment of Chlorella protothecoides chloroplasts for illumina genome sequencing.
With the increasing world demand for biofuel, a number of oleaginous algal species are being considered as renewable sources of oil. The paucity of algal chloroplast genome sequences has been an important constraint to chloroplast transformation and for studying gene expression in TAGs pathways. In this study, the intact chloroplasts were released from algal cells using sonication followed by sucrose gradient centrifugation, resulting in a 2.
This is the first report of an optimized protocol that uses a sonication step, followed by sucrose gradient centrifugation, to release and enrich intact chloroplasts from a microalga C. The approach is expected to guide chloroplast isolation from other oleaginous algal species for a variety of uses that benefit from enrichment of chloroplasts, ranging from biochemical analysis to genomics studies. Enhanced lipid accumulation and biodiesel production by oleaginous Chlorella protothecoides under a structured heterotrophic-iron II induction strategy.
A structured heterotrophic-iron II induction HII strategy was proposed to enhance lipid accumulation in oleaginous Chlorella protothecoides.
HII-induced cells produced significantly elevated levels of The lipid contents and plant lipid-like fatty acid compositions exhibit the potential of HII-induced C.
Furthermore, 31 altered proteins in HII-induced algal cells were successfully identified. These differentially expressed proteins were assigned into nine molecular function categories, including carbohydrate metabolism, lipid biosynthesis, Calvin cycle, cellular respiration, photosynthesis, energy and microaglas, protein biosynthesis, regulate and defense, and unclassified.
Analysis using the Kyoto encyclopedia of genes and genomes and gene ontology annotation showed that malic enzyme, acyltransferase, and ACP were key metabolic checkpoints found to modulate lipid accumulation in C. The results provided possible applications of HII cultivation strategy in other microalgal species and new possibilities in developing genetic and metabolic engineering microalgae for desirable lipid productivity.
Redistribution of metabolic fluxes in Chlorella protothecoides by variation of media nitrogen concentration.
microalga chlorella protothecoides: Topics by
Full Text Available In this study, the Elementary Metabolite Unit EMU algorithm was employed to calculate intracellular fluxes for Chlorella protothecoides using previously generated growth and mass spec data. Phosphoenolpyruvate carboxylase PEPCase activity was higher during nitrogen-sufficient growth.
The glyoxylate shunt was found to be partially active in both cases, indicating the nutrient nature has an impact on flux distribution. It was found that the total NADPH supply within the cell remained almost constant under both conditions. In summary, algal cells substantially reorganize their metabolism during the switch from carbon-limited nitrogen-sufficient to nitrogen-limited carbon-sufficient growth. The lab study was performed to observe the Pb II exposure induced changes. Results of the observations show: This research provides a basic understanding of Pb II toxicity to aquatic organisms.
Conteo de microalgas
Full Text Available To elucidate the lutein biosynthesis pathway in the lutein-producing alga, Chlorella protothecoides CS, the -carotene desaturase gene zds was isolated from Chlorella protothecoides using the approach of rapid amplification of cDNA ends. Sequence homology research indicated that the nucleotide and putative protein had sequence identities of Phylogenetic analysis demonstrated that the ZDS from C.
In addition, we also found that the zds gene expression was upregulated in response to light. Optimization of biodiesel production from Chlorella protothecoides oil via ultrasound assisted transesterification. Full Text Available There is a growing interest in biodiesel as an alternative fuel for diesel engines because of the high oil prices and environmental issues related to massive greenhouse gas emissions.
Nowadays, microalgal biomass has become a promising biodiesel feedstock. However, traditional biodiesel production from microalgae consumes a lot of energy and solvents. It is necessary to use an alternative method that can reduce the energy and alcohol consumption and save time.
In this study, biodiesel production from Chlorella protothecoides oil by ultrasound assisted transesterification was conducted and effects of reaction parameters such as methanol: The transesterification reactions were carried out by using methanol as alcohol and potassium hydroxide as the catalyst.
The highest methyl ester production was obtained under the conditions of 9: The results showed that ultrasound-assisted transesterification may be an alternative and cost effective way to produce biodiesel efficiently.
Conteo de microalgas | conteo de microalgas al microscopio | Fundación Ciencia Joven | Flickr
In this study, biodiesel production from microalgal oil by microwave-assisted transesterification was carried out to investigate its efficiency. Transesterification reactions were performed by using Chlorella protothecoides oil as feedstock, methanol, and potassium hydroxide as the catalyst. Biomass and lipid production of Chlorella protothecoides under heterotrophic cultivation on a mixed waste substrate of brewer fermentation and crude glycerol. Biomass and lipid accumulation of heterotrophic microalgae Mlcroalgas protothecoides by supplying mixed waste substrate of brewer fermentation and crude glycerol were investigated.
The biomass concentrations of the old and the new C.
The accumulated lipid productivities of the old and the new C. Our result highlights a new approach of mixing carbon-rich and nitrogen-rich wastes as economical and practical alternative substrates for biofuel production. In this study, the aquatic unicellular alga Chlorella protothecoides C.
A real-time PCR assay suggests the changes in transcript abundances of three photosynthetic-related genes. As for 28 d treatments, the three genes displayed similar inhibitory trend.
Characterization of microalga Chlorella as a fuel and its thermogravimetric behavior. Microalgae are photosynthetic microorganisms living in marine or freshwater environment.
In this study, samples of Chlorella spp. Both microalgae samples were characterized in terms of elemental composition CHONS and P and thermogravimetric behavior. Activation energy, reaction order and pre-exponential factor were calculated for the single step conversion mechanism of 1 g of Chlorella spp.
Calculated kinetic parameters, given as intervals of several determinations, resulted to be: Thermogravimetric analysis of the gasification of microalgae Chlorella vulgaris. The gasification of microalgae Chlorella vulgaris under an atmosphere of argon and water vapor was investigated by thermogravimetric analysis. The data were interpreted by using conventional isoconversional methods and also by the independent parallel reaction IPR model, in which the degradation is considered to happen individually to each pseudo-component of biomass lipid, carbohydrate and protein.
The IPR model allows obtaining the kinetic parameters of the degradation reaction of each component. Three main stages were observed during the gasification process and the differential thermogravimetric curve was satisfactorily fitted by the IPR model considering three pseudocomponents. The comparison of the activation energy values obtained by the methods and those found in the literature for other microalgae was satisfactory.
Quantification of reaction products was performed using online gas chromatography. The major products detected were H2, CO and CH4, indicating the potential for producing fuel gas and syngas from microalgae.
Anisotropic transport of microalgae Chlorella vulgaris in microfluidic channel. In this work, we study the regional dependence of transport behavior of microalgae Chlorella vulgaris inside microfluidic channel on applied fluid flow rate. The microalgae are treated as spherical naturally buoyant particles.
Deviation from the normal diffusion or Brownian transport is characterized based on the scaling behavior of the mean square displacement MSD of the particle trajectories by resolving the displacements in the streamwise flow and perpendicular directions. The channel is divided into three different flow regions, namely center region of the channel and two near-wall boundaries and the particle motions are analyzed at different flow rates.
We use the scaled Brownian motion to model the transitional characteristics in the scaling behavior of the MSDs. We find that there exist anisotropic anomalous transports in all the three flow regions with mixed sub-diffusive, normal and super-diffusive behavior in both longitudinal and transverse directions.